| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125277 |
| Chromosome: | chromosome 8 |
| Location: | 3898962 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378150 | GLD2 | Glucose-6-phosphate dehydrogenase; (1 of 2) 1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+)) / GPD | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAAGTCGAGCGCAAGCCGACCTCAAGTCT |
| Internal bar code: | ACGGTGCTTCATTACTGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 99.7361 |
| LEAP-Seq n confirming: | 2646 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTAGGCAAGTACGAGGG |
| Suggested primer 2: | GACCCAGAAGACTACGAGCG |