| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125291 |
| Chromosome: | chromosome 12 |
| Location: | 9198235 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541904 | (1 of 1) PF08755//PF13369 - Hemimethylated DNA-binding protein YccV like (YccV-like) // Transglutaminase-like superfamily (Transglut_core2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGAGGGATGATAGCGATGTGGATGCGA |
| Internal bar code: | CGAGGCGTCAAGTCTTAGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 90.9836 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATACGTCAGGCCACACA |
| Suggested primer 2: | CTCAGCTCCTCCCAGTCAAC |