| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125305 |
| Chromosome: | chromosome 6 |
| Location: | 1715155 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261500 | CPLD58 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR11260:SF136 - THIOREDOXIN FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTATGCAACTCAACACTCCCGTCAAT |
| Internal bar code: | CTGCCGCTAGCGTATGGAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 530 |
| LEAP-Seq percent confirming: | 99.8079 |
| LEAP-Seq n confirming: | 5196 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCAGTGTACCAGCCTCC |
| Suggested primer 2: | AAACGAATGTGTGCGTGTGT |