| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125331 |
| Chromosome: | chromosome 11 |
| Location: | 3349767 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAGGTTTGCTCTCCTGCATGCATGCGGC |
| Internal bar code: | CGATGTCGGCACGGACAAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 795 |
| LEAP-Seq percent confirming: | 97.6492 |
| LEAP-Seq n confirming: | 5857 |
| LEAP-Seq n nonconfirming: | 141 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCAGGCAAGAAAGGCAG |
| Suggested primer 2: | GCACTCACAGCCTCAATCAA |