Insertion junction: LMJ.RY0402.125336_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.125336
Chromosome:chromosome 6
Location:7218088
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g297904 (1 of 1) K10863 - aprataxin [EC:3.-.-.-] (APTX) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGGGGCTGATGGACGGCTTGGTGGGCT
Internal bar code:AGACCCCTTCAGTCGTCGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:440
LEAP-Seq percent confirming:9.91736
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:109
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCCATGACGGAGGTGAAG
Suggested primer 2:CGTTGTAGCACCCACAACAC