| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125343 |
| Chromosome: | chromosome 6 |
| Location: | 942503 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255450 | MAT3,RB | (1 of 1) K04681 - retinoblastoma-like protein 1 (RBL1); Retinoblastoma protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGCTTATGCATGCGAGGGGACCGCCA |
| Internal bar code: | AGCACAGACTAGTGCGTACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAGGAAGAGGAGGTCG |
| Suggested primer 2: | TGTATGTCGAGAGACAGGCG |