Insertion junction: LMJ.RY0402.125349_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.125349
Chromosome:chromosome 11
Location:3789656
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g483351 PHC44 Putative pherophorin-chlamydomonas homolog; (1 of 1) IPR003072//IPR003882//IPR024616 - Orphan nuclear receptor, NOR1 type // Pistil-specific extensin-like protein // Pherophorin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGTGTGCGGCTGCTCACCCTATCGGCT
Internal bar code:TTGTTAGATTAACCGGAGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:99.7029
LEAP-Seq n confirming:3692
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GACACCGACGCGTAACCTAT
Suggested primer 2:CAACGCAGATTTCGAAGTGA