| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125371 |
| Chromosome: | chromosome 17 |
| Location: | 988790 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703176 | MSD5 | (1 of 5) K04564 - superoxide dismutase, Fe-Mn family (SOD2); Mn superoxide dismutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCTGTCTACAGCTGGGGTCCCGCACC |
| Internal bar code: | GTTAATACTGGCGAGTTATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 96.4654 |
| LEAP-Seq n confirming: | 655 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAATCCCGCCCACTAAGTA |
| Suggested primer 2: | CGTAGAAGGAGCTCACCTGG |