Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.125379 |
Chromosome: | chromosome_14 |
Location: | 3759817 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g632350 | FAP138 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATGCGGTATGCGCAGGCATGCCCGTACCA |
Internal bar code: | CGGTCATCCGTCCCAGGGGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 240 |
LEAP-Seq percent confirming: | 98.1982 |
LEAP-Seq n confirming: | 109 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGTGTCAAGTCACCAGCA |
Suggested primer 2: | ACAAGATATTGGTGCAGCCC |