Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.125381 |
Chromosome: | chromosome 12 |
Location: | 1214583 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g489500 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGGACAGCATCGGCACTACTGCCGAAG |
Internal bar code: | GACCCGCATCAGGAAATCGACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 820 |
LEAP-Seq percent confirming: | 98.4127 |
LEAP-Seq n confirming: | 1488 |
LEAP-Seq n nonconfirming: | 24 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGCAGGTCTAATGCTTGGC |
Suggested primer 2: | AAGCGACATGGCTGAAGACT |