| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125388 |
| Chromosome: | chromosome 12 |
| Location: | 4679301 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523276 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGGAGTGCATGCTCCGCGGCCCGTCC |
| Internal bar code: | TGGTTCAGTGCGGAAAAGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 97.4839 |
| LEAP-Seq n confirming: | 1821 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCGTCCCCAAACACC |
| Suggested primer 2: | TAGAGCGACACCACTGCATC |