Insertion junction: LMJ.RY0402.125399_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.125399
Chromosome:chromosome 3
Location:9165345
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g212641 (1 of 16) 2.3.1.48 - Histone acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCATGTCACTGCTCGTCCCCATGCAAA
Internal bar code:TGGGAGTGCCAAGACTAGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:922
LEAP-Seq percent confirming:98.4579
LEAP-Seq n confirming:3767
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AACCGTGGTTTTGTCTGAGG
Suggested primer 2:CTCCGGGTTGTGCAACTTAT