| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125399 |
| Chromosome: | chromosome_3 |
| Location: | 9165345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g212641 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGCATGTCACTGCTCGTCCCCATGCAAA |
| Internal bar code: | TGGGAGTGCCAAGACTAGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 98.4579 |
| LEAP-Seq n confirming: | 3767 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGTGGTTTTGTCTGAGG |
| Suggested primer 2: | CTCCGGGTTGTGCAACTTAT |