Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.125415 |
Chromosome: | chromosome 6 |
Location: | 5235785 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g282250 | NAT18 | N-acetyltransferase; (1 of 1) 2.3.1.48//2.3.1.88 - Histone acetyltransferase // Peptide alpha-N-acetyltransferase / Protein N-terminal acetyltransferase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACACATAGCTGCGGGGAGGGAGGGGAA |
Internal bar code: | GACGCACTGGCCGACATGTGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 142 |
LEAP-Seq percent confirming: | 90.3437 |
LEAP-Seq n confirming: | 552 |
LEAP-Seq n nonconfirming: | 59 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACACACGCGCACACTCATA |
Suggested primer 2: | ACCACGCTTGTAGGTATGGC |