Insertion junction: LMJ.RY0402.125415_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125415
Chromosome:chromosome 6
Location:5235785
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g282250 NAT18 N-acetyltransferase; (1 of 1) 2.3.1.48//2.3.1.88 - Histone acetyltransferase // Peptide alpha-N-acetyltransferase / Protein N-terminal acetyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACACATAGCTGCGGGGAGGGAGGGGAA
Internal bar code:GACGCACTGGCCGACATGTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:142
LEAP-Seq percent confirming:90.3437
LEAP-Seq n confirming:552
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TACACACGCGCACACTCATA
Suggested primer 2:ACCACGCTTGTAGGTATGGC