Insertion junction: LMJ.RY0402.125428_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125428
Chromosome:chromosome_10
Location:4629818
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre10.g452950 TRP1 Transient receptor potential ion channel protein antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CGCAGCTGAAGGCCCCCCCGGCAGAGGGTG
Internal bar code:GCCAGTGTCCGGATAACGACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:41
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGGACAAGGAAGAAAGA
Suggested primer 2:GACACATCGAACATTGCACC