Insertion junction: LMJ.RY0402.125503_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.125503
Chromosome:chromosome 9
Location:1751089
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396550 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGGCCAATGCGAGAGGGTGCGTGTTTA
Internal bar code:CGTGGAAGACTGTAACACGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:723
LEAP-Seq percent confirming:99.6234
LEAP-Seq n confirming:1587
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACGTGTGTGGTTTATCG
Suggested primer 2:CTCAATTGCGTATGGCCTTT