Insertion junction: LMJ.RY0402.125504_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125504
Chromosome:chromosome 2
Location:398373
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g076000 LAT3,STK1,STPK1,SNRK2K (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2; Latrunculin-B sensitive 3 gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTAGGTTCAGGACTTCAGGTTCAGGTTT
Internal bar code:TCTCGGGTGCGCCGGAGCGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:608
LEAP-Seq percent confirming:99.6287
LEAP-Seq n confirming:1610
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGTGTGGTTACAAGGTG
Suggested primer 2:AAATCGCAAGGATTCCAATG