Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.125535 |
Chromosome: | chromosome_17 |
Location: | 3900036 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g728300 | FAP226,CAL6 | Calpain cysteine protease and Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTAAACCGGACCGGGGGAAGAGTGGTCGGG |
Internal bar code: | TGATACCGTTTAATGAAATTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 619 |
LEAP-Seq percent confirming: | 96.8509 |
LEAP-Seq n confirming: | 1630 |
LEAP-Seq n nonconfirming: | 53 |
LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGCCAGGTAGTACGGTAG |
Suggested primer 2: | CGTCTTCGTTGGTTTTGGTT |