| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125545 |
| Chromosome: | chromosome_12 |
| Location: | 2901406 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g501900 | FFT2 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCCCTGTTGGCGTGCAATGCTGCTGCGT |
| Internal bar code: | CTCATCTAGGCCTGGATTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 97.2778 |
| LEAP-Seq n confirming: | 2287 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTACCGCCATCACGTTT |
| Suggested primer 2: | CTGGAACCCCTTTTCTCTCC |