Insertion junction: LMJ.RY0402.125545_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.125545
Chromosome:chromosome 12
Location:2901411
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g501900 FFT2 putative fructan fructosyltransferase; (1 of 2) PTHR31953//PTHR31953:SF1 - FAMILY NOT NAMED // BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV2-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTTTGTTGCGCTTGGCGTCCCGCCCGTG
Internal bar code:GACACGTCGGCTCCTAAGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:877
LEAP-Seq percent confirming:98.8451
LEAP-Seq n confirming:5820
LEAP-Seq n nonconfirming:68
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTACCGCCATCACGTTT
Suggested primer 2:CTGGAACCCCTTTTCTCTCC