| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125562 |
| Chromosome: | chromosome_16 |
| Location: | 1942653 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g656300 | DPR1 | Dihydrodipicolinate reductase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTCAAGCGCCAGTGCGTCGCCGCGGCCC |
| Internal bar code: | GAGTGCGACGGAGCCCGAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTGCTTTCACACAAGA |
| Suggested primer 2: | TTTATGTTGTCTTGCGCAGC |