Insertion junction: LMJ.RY0402.125575_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125575
Chromosome:chromosome 13
Location:3661928
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g588800 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTAGACGCCCTACGGCGGGCTGCGGAT
Internal bar code:TGTCGTGCTCCAGATCGGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:912
LEAP-Seq percent confirming:98.7052
LEAP-Seq n confirming:2287
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CACACCAAACAATGCAGACC
Suggested primer 2:GCCGGTGCTTGTAGTCAAAT