Insertion junction: LMJ.RY0402.125577_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125577
Chromosome:chromosome 13
Location:896443
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g567650 (1 of 1) 3.4.14.2 - Dipeptidyl-peptidase II / Quiescent cell proline dipeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTCCTGCACGCGCGCGAGGGAGGTATGC
Internal bar code:GTGAATTTAACAACTTCGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:740
LEAP-Seq percent confirming:69.5127
LEAP-Seq n confirming:5007
LEAP-Seq n nonconfirming:2196
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACACACCGT
Suggested primer 2:TAACTCTGAACCAATCCCCG