Insertion junction: LMJ.RY0402.125614_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.125614
Chromosome:chromosome 9
Location:2188634
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g393100 (1 of 8) PF01697 - Glycosyltransferase family 92 (Glyco_transf_92) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGCACCTACCTGCGCCATCGTGGTCGG
Internal bar code:GCTTATTTACGGGGCGGGTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:782
LEAP-Seq percent confirming:99.8556
LEAP-Seq n confirming:3458
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATGAATGTAACCCCCAC
Suggested primer 2:CTGCTAGGGTTGCTTCTGCT