| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125617 |
| Chromosome: | chromosome 6 |
| Location: | 3807876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278166 | PRL4 | Predicted extracellular protein; (1 of 1) PTHR10334:SF165 - PATHOGENESIS-RELATED PROTEIN-RELATED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCAATGTATGAATAACACATGTACTCA |
| Internal bar code: | TCCGTTTTGATGTGACAAAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.7832 |
| LEAP-Seq n confirming: | 3222 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTCACTAGCACGCTG |
| Suggested primer 2: | TGCATGCTCAGTTAAGACGG |