| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125637 |
| Chromosome: | chromosome 14 |
| Location: | 4105386 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGACACGGACTCGAGCAGCGACAGCGG |
| Internal bar code: | GCGCTCGCAACAAGGTTCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.7435 |
| LEAP-Seq n confirming: | 4667 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACCTGTCCCTGCTGCTT |
| Suggested primer 2: | GGGTAGCTGTACGGTTTGGA |