Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.125642 |
Chromosome: | chromosome_4 |
Location: | 2761644 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g223650 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TAGCGCACCTCCGAGGCAAACACATATTGG |
Internal bar code: | CACTTGTCTCAGAACGGGATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 919 |
LEAP-Seq percent confirming: | 98.1775 |
LEAP-Seq n confirming: | 1239 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTGCTGAGTATGTGCGTGG |
Suggested primer 2: | CCTGTGCAAACTGCAAAAGA |