| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125647 |
| Chromosome: | chromosome 16 |
| Location: | 5749280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678650 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTCCGTGCTGGCCTTGTGCTCGGCCAG |
| Internal bar code: | CCTGTTGTCTATCGCATGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 948 |
| LEAP-Seq percent confirming: | 99.6852 |
| LEAP-Seq n confirming: | 5700 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGATTCAAGTTCCTGTGT |
| Suggested primer 2: | ACAGTTCTGGGACTGCTGCT |