Insertion junction: LMJ.RY0402.125649_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125649
Chromosome:chromosome 9
Location:6243784
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g406350 (1 of 1) K15287 - solute carrier family 35, member F1/2 (SLC35F1_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACACCTCGCCCGCATGCCCAACGACAA
Internal bar code:TCCTCGCTTGAACATCTGTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:99
LEAP-Seq percent confirming:99.7333
LEAP-Seq n confirming:374
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACCCTCTACCCACTTC
Suggested primer 2:GTGACGCCATCATCATCAAC