| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125664 |
| Chromosome: | chromosome 10 |
| Location: | 1713846 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430450 | (1 of 1) 3.5.1.10 - Formyltetrahydrofolate deformylase / Formyltetrahydrofolate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGACACTTGATTCAAACCTCGCACCCTG |
| Internal bar code: | GGGCTTTGTTTCGGGTCCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 67.5 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAGAAGCACCCTGAGC |
| Suggested primer 2: | TCACAGAGGAGCAGGTGTTG |