Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.125665 |
Chromosome: | chromosome_11 |
Location: | 2737500 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g477100 | FAP97 | Flagellar Associated Protein, serine endopeptidase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATGTCCGGAGAAAATCCTAGGGAGGTCAG |
Internal bar code: | GGCGATGTTGCTTTTTTAGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 878 |
LEAP-Seq percent confirming: | 99.184 |
LEAP-Seq n confirming: | 1337 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCTTTGCGCAATGTTTTCC |
Suggested primer 2: | GAAGGCAGTTGAGGACAAGC |