Insertion junction: LMJ.RY0402.125665_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.125665
Chromosome:chromosome_11
Location:2737500
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre11.g477100 FAP97 Flagellar Associated Protein, serine endopeptidase sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GATGTCCGGAGAAAATCCTAGGGAGGTCAG
Internal bar code:GGCGATGTTGCTTTTTTAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:878
LEAP-Seq percent confirming:99.184
LEAP-Seq n confirming:1337
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTTTGCGCAATGTTTTCC
Suggested primer 2:GAAGGCAGTTGAGGACAAGC