| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125667 |
| Chromosome: | chromosome 1 |
| Location: | 2550889 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014751 | (1 of 7) PF14555 - UBA-like domain (UBA_4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGCAGAGCGCCATGCGAGGTGTACGCG |
| Internal bar code: | GGTTTCACCGGAACGATGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 99.5261 |
| LEAP-Seq n confirming: | 210 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGAGAACTGTAGGCGGCG |
| Suggested primer 2: | CCCAAACTAGCCACACCCTA |