| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125679 |
| Chromosome: | chromosome 6 |
| Location: | 6847117 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295250 | (1 of 1) K07252 - dolichyldiphosphatase (E3.6.1.43) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCTTGGGTTAACCCGAAGCTATGCTA |
| Internal bar code: | ATGACAAAGTCCCGGGCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.327 |
| LEAP-Seq n confirming: | 738 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAGCACTTTGACAGCGG |
| Suggested primer 2: | ACTGCCCAGTGCCATAAAAC |