| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125684 |
| Chromosome: | chromosome 12 |
| Location: | 5997834 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534961 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCAGCGGCTTCAGTAACCAGGGCGCAC |
| Internal bar code: | GCAAATTCTTTGGCGTTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.7186 |
| LEAP-Seq n confirming: | 1063 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATCACGGGTCCTTACA |
| Suggested primer 2: | TGCTTTCTGGTTCAGTCACG |