| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125697 |
| Chromosome: | chromosome 1 |
| Location: | 5233310 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036400 | SDR3 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.313 - Sulfoacetaldehyde reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCAGCTGGGCAGCTTCCCCGCAGCCGG |
| Internal bar code: | TGGATATTAATCTCGTTTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.426 |
| LEAP-Seq n confirming: | 3984 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGATGTTGACGATGTGC |
| Suggested primer 2: | AGCAAGCAGAAGGTTCCAAA |