Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.125702 |
Chromosome: | chromosome_2 |
Location: | 750347 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g078550 | FAP210 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCGATGGAGGTGATGTAGGAGGTGGCAAGG |
Internal bar code: | CTTCGCCTTGATCCGGGATTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 848 |
LEAP-Seq percent confirming: | 99.3909 |
LEAP-Seq n confirming: | 1795 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTCCAACTACATGGCCTGG |
Suggested primer 2: | ATTGGTTGCATGAGTAGGGC |