| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125712 |
| Chromosome: | chromosome 16 |
| Location: | 6281941 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674700 | AGH1 | ADP-ribosyl glycohydrolase; (1 of 1) 3.2.2.24 - ADP-ribosyl-[dinitrogen reductase] hydrolase / Dinitrogenase reductase activating glycohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGCCTCCTGACCTGGCTGCCTGCTAA |
| Internal bar code: | TGACTCGAGCCTATGTTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 99.3534 |
| LEAP-Seq n confirming: | 3995 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCACTGGGACGTCAACC |
| Suggested primer 2: | CGTGGGTTTTGCCTACATCT |