| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125718 |
| Chromosome: | chromosome 2 |
| Location: | 2871184 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094801 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCAGGGAAAGAGGAGGGGAATGGGGCT |
| Internal bar code: | TGTCATTCCACCAGTTCCCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 99.0016 |
| LEAP-Seq n confirming: | 3768 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGGAGTTGTGAATTGTG |
| Suggested primer 2: | GGCTGAAGCACCTCTGGTAG |