| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125719 |
| Chromosome: | chromosome 17 |
| Location: | 3725355 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727300 | NFO2 | (1 of 3) 1.3.1.42 - 12-oxophytodienoate reductase; NADH:flavin oxidoreductase/NADH oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCCTGTGCCTCATGCACCCTTCCCCC |
| Internal bar code: | GCTGGCCATCATGCCCATCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 99.0366 |
| LEAP-Seq n confirming: | 514 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGCTCCAAACAACACA |
| Suggested primer 2: | TACCTGCACACCTCCCTACC |