Insertion junction: LMJ.RY0402.125732_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.125732
Chromosome:chromosome 16
Location:1146808
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g650300 HTR39,HTR25 Histone H3; (1 of 35) K11253 - histone H3 (H3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGGCGCGGCGGGGCGTGTGCTACGTGCT
Internal bar code:ACCCTTGGGCTAGAGCCCCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:202
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:151
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTCCTCGTAGATCAGGC
Suggested primer 2:TCCTTCAGGAGCAAACGAGT