| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125746 |
| Chromosome: | chromosome 2 |
| Location: | 285392 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075050 | PTA1 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCTTCTCTATGTCCGCCCAAAATGTAG |
| Internal bar code: | AAGGTTATAGTTGCGTTATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 99.2126 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACCCTGATGTCGAGTT |
| Suggested primer 2: | ACGCGCTTGATATTCAGCTT |