Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.125754 |
Chromosome: | chromosome 7 |
Location: | 3427356 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g335250 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGTCAACAACACCGCAACTGCGCATC |
Internal bar code: | TGATCCGTTGCACCGTTCCCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 763 |
LEAP-Seq percent confirming: | 97.561 |
LEAP-Seq n confirming: | 40 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCCCTAGCGACAACACACT |
Suggested primer 2: | CCGTGTAATGAACATTTGCG |