| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125762 |
| Chromosome: | chromosome 3 |
| Location: | 384010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144867 | (1 of 1) K18624 - macrophage erythroblast attacher (MAEA, EMP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCAGCAATGCGGTGCCGGGGTTTATGTG |
| Internal bar code: | CAGCGAAAGTGGAACCAAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 98.9499 |
| LEAP-Seq n confirming: | 4052 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGCACACAGCACGAAC |
| Suggested primer 2: | GAACGGGTTCTGTTCTCAGC |