| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125772 |
| Chromosome: | chromosome 7 |
| Location: | 3644690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337125 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGTCCGCGTGTGAAACGCGTCAGCGTT |
| Internal bar code: | CGTGCGGTCGGGTCTTCCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 70.0704 |
| LEAP-Seq n confirming: | 398 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGCTTACCCGTCCAAT |
| Suggested primer 2: | GAGGAAGCTGACTGGGACTG |