| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125781 |
| Chromosome: | chromosome 3 |
| Location: | 5378723 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184500 | (1 of 2) PTHR33925:SF3 - PROTEIN ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, CHLOROPLASTIC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGGGCGAGGCGGACAGTTCGGGGTTGG |
| Internal bar code: | TTAGATAGACAAAGTTTTAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 99.848 |
| LEAP-Seq n confirming: | 1314 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTACCAGTTCCTGTGC |
| Suggested primer 2: | AGCTCGCTGTCCTTGAATGT |