| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125815 |
| Chromosome: | chromosome 5 |
| Location: | 1075282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246150 | CYG13 | Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACTCAAGGCTTGATATATAGAGACGTTC |
| Internal bar code: | ATTATTACCGGGGCGCCCTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 96.9027 |
| LEAP-Seq n confirming: | 1095 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATAATGTCGAAGCTCA |
| Suggested primer 2: | ATGGATTCGTTGAACAAGGC |