| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125820 |
| Chromosome: | chromosome 6 |
| Location: | 5632234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285900 | FAP396 | (1 of 2) IPR000104//IPR003590 - Antifreeze protein, type I // Leucine-rich repeat, ribonuclease inhibitor subtype; Flagellar Associated Protein 396 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAGGCCACCTGCATAGCACAGCTCCAG |
| Internal bar code: | GATTGGTTGGCGACCTGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 99.5496 |
| LEAP-Seq n confirming: | 221 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGCGCTATCCTATGAA |
| Suggested primer 2: | TTCGTGTAATTGGTGTGCGT |