| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.125837 |
| Chromosome: | chromosome 4 |
| Location: | 127425 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216774 | (1 of 2) IPR000601 - PKD domain | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGAAATCAGGCTGGTGCCCCGCGTACGC |
| Internal bar code: | GGTCCGCGAACACGGCTAACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 97.7851 |
| LEAP-Seq n confirming: | 5033 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATTTTGTTTGCGGCCTG |
| Suggested primer 2: | TGTGTGTGCTCACCTGGAAT |