| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.125851 |
| Chromosome: | chromosome 12 |
| Location: | 1635278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486208 | Found in Cr and Volvox but not strongly conserved elsewhere | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGACAGCAACGTATTGCTATGCCAGGT |
| Internal bar code: | GTCTTCGCCCCGCTCCGGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 98.5573 |
| LEAP-Seq n confirming: | 1298 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGTGAGCTTCCCACTTG |
| Suggested primer 2: | GTGTTTCTGCGTACGACCCT |