| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.125851 |
| Chromosome: | chromosome 12 |
| Location: | 1635297 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486208 | Found in Cr and Volvox but not strongly conserved elsewhere | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCGATGCGGGCGCCTTCGTGCCCAGC |
| Internal bar code: | TGGTGTGAGTATTGTGTACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 98.7421 |
| LEAP-Seq n confirming: | 471 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGTGAGCTTCCCACTTG |
| Suggested primer 2: | GTGTTTCTGCGTACGACCCT |