| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.125864 |
| Chromosome: | chromosome_11 |
| Location: | 2703101 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476850 | DRC4,PF2 | Component of dynein regulatory complex | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGCTAGTTCCTTTCCAGACGACGTGCATG |
| Internal bar code: | CATGGACACGCCCCATTGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 99.6256 |
| LEAP-Seq n confirming: | 6120 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTCACAGAGTTCGGCAT |
| Suggested primer 2: | ACTTCCCACCATGGACTCTG |